Professor ZHOU Shi-yuan, has extensive professional experience working on the treatment of infertility. According to the steps of "regulating the menstrual cycle, stimulating follicular development, promoting ovulation at right time and choosing the best time to get pregnant", it has been effective increasing the conception rate for clinic patients with infertility by accurately ovulation induction. To make treatment for female infertility, Professor ZHOU initially makes clear causes and pathogenesis of infertility and lays emphasize on regulating menstrual cycle by stages: activating blood circulation to dredge collaterals during menstruation; tonifying the Kidney Qi and replenishing vital essence after menstruation; warming Yang to dredge collaterals and promoting blood circulation during ovulation; based on the principle of promoting both Yin and Yang and tonifying Kidney Yang, making treatments in different stages and putting more emphasis on regulating the functions of liver, spleen and kidney after ovulation. Then she is skilled in integrated treatment of Chinese and Western medicine. As the main feature, she uses herbal medicine with acupuncture to stimulate follicular development and increase the thickness of the endometrial layer at the best time. Finally, she emphasizes to accurately grasp the best time to get pregnant, that is, when the endometrial thickness should reach 8mm or more and the follicles should be between 18 and 25 mm in size for releasing of the egg under the pregnancy guidance. In conclusion, the purpose of this paper is to summarize Professor ZHOU's experience of promotion ovulation and assisted pregnancy which offers reference for clinical treatment for infertility.